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200-Hour Physician Course

Fundamentals of Chromatography for Technetium-99m Radiopharmaceuticals

Program Description

The goal of this learning activity is to provide a basic overview of theories, techniques, and practical aspects applicable to testing Tc-99m labeled radiopharmaceuticals for radiochemical purity using chromatography. 

This is a knowledge based activity for pharmacists, technologists, and technicians.  Learning Objectives: 

  1. Explain the rationale for testing radiopharmaceuticals for radiochemical purity.
  2. Explain the principles of radiochemical purity testing using chromatography.
  3. List types of stationary (solid) phase materials as well as the care, storage, and use of each.
  4. List types of mobile (solvent) phase materials as well as care, storage, and use of each.
  5. Explain proper strip preparation and labeling.
  6. For commonly dispensed Tc-99m radiopharmaceuticals:
      -  Name the solid and mobile phases used in chromatography.
      -  List the expected location of anticipated radiochemical species on the solid phase including potential   impurities found in Tc-99m labeled radiopharmaceuticals
      -  List the acceptable limits of labeled radiopharmaceutical for product release (both USP and in-house limits).
  7. Calculate the percentage of the labeled (bound) radiopharmaceuticals.
  8. List at least four procedural checks to be made when chromatography identifies a “failed” product.
  9. Given a chromatographic scenario, hypothesize a logical explanation of events.
  10. Explain the impact that substandard radiopharmaceuticals have on patient care.
  11. Explain methods necessary to validate alternate chromatography procedures.


Kristina Wittstrom, BCNP
University of New Mexico College of Pharmacy
Albuquerque, NM
ACPE accreditation for this program has expired. 

Also available

Drug-Radiopharmaceutical Interactions CE  Expired

Current Practices in Renal Imaging - Expired

Interested in the development of a continuing education program?  Contact Nicki Hilliard at 501-686-6398


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